![]() ![]() ![]() We also show 10–70% more T cells (total) within 30 µm of a neighboring T helper cell in 3D vs 2D. Comparing the 3D reconstructed cellular data with the 2D data, we show significantly shorter distances between immune cells and vascular endothelial cells (56 µm in 3D vs 108 µm in 2D). We demonstrate MATRICS-A in 26 serial sections of fixed skin (stained with 18 biomarkers) from 12 donors aged between 32–72 years. Here we show a workflow for 3D reconstruction of multiplexed sequential tissue sections: MATRICS-A ( Multiplexed Im age Three-D Reconstruction and Integrated Cell Spatial - Analysis). 2D spatial cell analysis in a single tissue section may be limited by cell numbers and histology. Mapping the human body at single cell resolution in three dimensions (3D) is important for understanding cellular interactions in context of tissue and organ organization. ![]()
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